Barney's Lake Nature Preserve encompasses a rich variety of habitats including open meadows, hardwood forest, peatlands, sedge meadows, and cedar swamp. The trail system is on the eastern side the preserve, across the road from Barney's Lake. The trail begins in an open meadow that is an old homestead. Most of the trails are on the upland portions of the property. Two-tracks that were once logging roads intersect the property, providing more opportunities to explore the property.
The parking area is located on the eastern side of Barney's Lake Road, across from the boat launch and lookout area. The preserve includes 2,200 feet on Barney's Lake.
To get to the preserve from Port of St. James, take Kings Highway south 1¼ miles to Barney's Lake Rd. North. Turn right and continue 2½ miles. The preserve is on both sides of the road.